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KMID : 0603520020070020094
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Journal of Korean Association of Cancer Prevention
2002 Volume.7 No. 2 p.94 ~ p.100
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Identification and Functional Analysis of Egr-l-interacting Proteins Involved in Vasculogenesis During Early Stage of Development
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Bae Myung-Ho
Bae Moon-Kyoung
Jeong Joo-Won
Kim Se-Hee
Jeong Chul-Ho
Bae Soo-Kyung
Kim Kyu-Won
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Abstract
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Egr-I is a zinc finger transcription factor and one of immediate early response genes. Egr-l is upregulated in response to a wide variety of mitogenic and nonmitogenic stimuli, including peptide growth factors, shear stress, urea, hypotonicity and hypoxia. To identify novel Egr-l interacting protein, we performed yeast two-hybrid screening using Egr-l baits. In this study, we identified that proteasome subunit αtype 3 (PSMA3) and protein inhibitor of activated STAT protein (PIASy) interacts specifically with Egr-l protein by yeast two-hybrid screening. In addition, we found that the stability of Egr-l was increased under hypoxic condition. these results suggested the importance of Ｏ₂tension in the degradation processes of Egr-l.
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KEYWORD
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Egr-1, Two-hybrid, Proteasome subunit α type 3, ProtehI inhibitor of activated STAT protein y, Hypoxia
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